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Revista mexicana de fitopatología

versão On-line ISSN 2007-8080versão impressa ISSN 0185-3309

Rev. mex. fitopatol vol.36 no.3 Texcoco Out./Dez. 2018

https://doi.chrissiemanby.com/10.18781/r.mex.fit.1803-4

Early morphological development of sclerotia of Sclerotinia sclerotiorum in the presence of potassium bicarbonate


Claudia Ordóñez-Valencia1 

Ronald Ferrera-Cerrato1 

Alejandro Alarcón1 * 

Laura V. Hernández-Cuevas2 

John Larsen3 


1 Área de Microbiología, Posgravì chưng de Edafología.Colegio de Postgraduados. Carretera México-Texcoteo Km 36.5. Montecillo, Texcoteo,Estado de Méxiteo, CPhường. 56230 México

2 Centro de Investigación en Genética yAmbiente. Universidad Autónoma de Tlaxcala. Autopista Texmelucan-Tlaxcala Km10.5. Ixtacuixtla CPhường. 901đôi mươi, Tlaxcala, México

3 Institukhổng lồ de Investigaciones en Ecosistemas ySustentabilidad, Universidad Nacional Autónoma de Méxiteo. Antigua Carretera aPátzcuaro No.8701, Colonia Ex Hacienda de San José de la Huerta, Morelia,Michoacán, CP.. 58190 Méxiteo.

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Abstract:

Sclerotinia sclerotiorum is a pathogen of great economic importance that causes significant losses in various crops. Control of the pathogene is difficult since this fungus forms resistant sclerotia that can survive in the soil for many years. This study evaluated the morphological development of S. sclerotiorum sclerotium primordia by using the Riddell technique, và different concentrations of potassium bicarbonate (KHCO3). The formation of primordia began from hyphae. However, as the concentration of KHCO3 increased, morphological changes were observed in the initiation phase of the sclerotia, as well as in the inhibition of their development when using a 50 mM concentration of KHCO3. This chemical compound modifies the morphology và inhibits the development of sclerotia in their initial stages; hence it may offer potential as an alternative sầu lớn synthetic fungicides for the control of plant diseases caused by S. sclerotiorum.

Key words: antifungal agent; morphogenesis; inhibition; microscopy


Resumen:

Sclerotinia sclerotiorum es un patógeno de suma importancia económica quecausa grandes pérdidas en vargame ios cultivos. Controlar este patógeno es difícilporque forma estructuras de resistencia llamadas esclerocgame ios que puedenmantenerse viables en el suelo por muchos años. Este estudio evaluó eldesarrollo morfológiteo de los primordgame ios de esclerocquả táo de S.sclerotiorum utilizando la técnica de Riddell, y diferentesconcentraciones de bicarbonato de potasio (KHCO3). La formación delos primordgame ios de los esclerocios inició a partir de hifas; sin embargo,conforme las concentraciones de KHCO3 incrementaron, se observaroncambgame ios morfológicos en la fase de iniciación de los esclerocgame ios, así como en lainhibición de su desarrollo al utilizar una concentración de 50 mM deKHCO3. Este compuesto químico modifica la morfología e inhibe eldesarrollo de esclerocios en su fase inicial y, por tanto, podría utilizarsecomo alternativa a los fungicidas sintéticos para controlar enfermedades deplantas causadas por S. sclerotiorum.

Palabras clave: agente antifúngico; morfogénesis; inhibición; microscopia


White mold is caused by Sclerotinia sclerotiorum, a fungus that belongs to the family Sclerotiniaceae. This is a destructive fungal pathoren for many agricultural crops such as sunflower, soybean, oilseed rape, bean, chickpea, canola, and onion (Hegedus and Rimmer, 2005: Bolton et al; 2006). It has little host specifithành phố, thus being able khổng lồ infect over 400 plant species, mainly dicotyledons (Fernanbởi et al., 2004; Hegedus and Rimmer, 2005). The environmental conditions that promote the fungal infection are high humidity and temperatures between 15 & 25 °C (Saharan & Mehta, 2008). Secretion of fungal enzymes such as cellulases và pectinases, that soften & degrade plant tissues are involved in the plant infection process (Fernando et al., 2004; Bolton et al., 2006), as well as production of oxalic acid, which has toxic effects on the tissue of the host (Hegedus & Rimmer, 2005). One characteristic of this pathogen is the formation of sclerotia, fungal structures of resistance and dispersal, which under favorable conditions can remain viable for several years in soils (Bae and Knudsen, 2007; Calvo and Cary 2015; Smith et al., 2015).

During the formation of sclerotia, three stages or phases have sầu been identified: 1) initiation: aggregation of hyphae, 2) development: hyphal growth for greater form size, và 3) maturation: creation of surface boundaries, internal consolidation & melanization (Le Tourneau, 1979; Rollins & Dickman, 2001; Bolton et al., 2006; Saharan & Mehta, 2008). The initiation và maturation stages may be influenced by abiotic factors such as photoperiod, temperature, oxygene, and nutrient availability (e.g. carbon sources), and the morphogenesis & further development of sclerotia started between 12 and 24 h of fungal growth (Hansberg và Aguirre, 1990).

The sclerotium is composed by three layers: a thichồng và pigmented outer layer, an intermediate & thin layer, & an internal trắng layer called the inner medulla (Punja và Damiani, 1996; Bardin và Huang, 2001). Depending on environmental conditions, sclerotia grow belowground in one of two ways: 1) by forming mycelium that potentially infects roots và causes rot & wilting of plant tissues, or 2) by producing apothecia, in which ascospores are produced và released, then infecting aerial plant chrissiemanby.comans (Humpherson-Jones and Cooke, 1977; Mónaco et al., 1998; Bolton et al., 2006).

Bicarbonates possess antimicrobial properties of wide spectrum, and their efficiency has been proven for controlling many plant pathogenic fungi (Bombelli and Wright, 2006; Arslan, 2015). The Environmental Protection Agency (EPA) of the United States also recognizes bicarbonates as innocuous and safe compounds for both human health & environment (Palmer et al., 1997; Bombelli & Wright, 2006), since their use may decrease the utilization of pesticides. Some studies have sầu shown that sodium, potassium & ammonium carbonates và bicarbonates inhibit the post-harvest growth of several fungal pathogens in fruits, vegetables and ornamental plants (Karabulut et al., 2003; Arslan et al., 2006; Jabnoun-Khiareddine et al., 2016). Bicarbonates alter the permeability of fungal membranes, inhibit the reactions of oxidative sầu phosphorylation, và exert toxic effects on the structures of the pathogene (Avis, 2007). However, their efficacy depends on the concentration (0.2-3%) và on the susceptibility of each microchrissiemanby.comanism. For instance, treatments with sodium carbonate and bicarbonate improved the control of the green mold caused by Penicillium digitatum Sacc. (Trichocomaceae), in citrus fruits (Smilanick et al., 1999). Sodium & potassium bicarbonates also reduced powdery mildew caused by Leveillula taurica (Lév.) Arnaud (Erysiphaceae) in peppers (Fallik et al., 1997), & decreased the conidiogenesis by Helminthosporium solani Durieu y Mont. (Pleosporaceae) (Olivier et al, 1998). In addition, Bombelli & Wright (2006), và Türkkan et al. (2017) observed the growth inhibition of Botrytis cinerea Pers. Fr. (Sclerotiniaceae) when exposed to lớn different bicarbonates with in vitro cultures. Plant disease control in carrot, cucumber & cantaloupe fruits has been also reported due khổng lồ the application of bicarbonates (Aharoni et al., 1997; Bombelli và Wright, 2006).

Overall, the inhibitory effects of potassium bicarbonate (KHCO3) on the growth of S. sclerotiorum, as well as on the germination & formation of new sclerotia have been described (Ordóñez-Valencia et al., 2009), but the effects of this chemical compound during early stages of sclerotia morphological development are not well understood. Thus, the ayên of this study was khổng lồ evaluate the effects of different doses of potassium bicarbonate on the early stages and primordia development of sclerotia by S. sclerotiorum via microscopic observations.


MATERIALS AND METHODS

In order to maintain the humidity in the Petri dishes, 10 mL of 10% glycerol were added. All Petri dishes were kept at room temperature conditions (~trăng tròn °C) & an approximate photoperiod of 12 h. Every day, the fungal growth was monitored under optical microscope (Leica CME, U.S.A.). Once the PDA-disk was fully covered with the fungal mycelium (approximately seven days of incubation), glycerol was replaced with a 10% formaldehyde solution, which was kept for 2 h for permanently fixing the fungal structures.

Later, the microscopic slide was removed from the Petri dish to prepare the fungal slides. The cover slip was carefully separated from the agar và placed on another clean slide on which a drop of the colorant cốt tông xanh in lactophenol was added. The next step was lớn remove sầu the PDA-disk from the original microscopic slide on which the colorant was also added, and a clean cover slip was immediately placed on it. In this way, four fungal preparations were obtained from each concentration of KHCO3, including the control without bicarbonate. Once the excess of colorant was removed, the stained fungal preparations were sealed with colorless nail polish, & evaluated under light microscope. The microscopic evaluations consisted on identifying the growth of the sclerotial primordia in each concentration of KHCO3. For this, an optical microscope (OLYMPUS BX51, Japan) was utilized for taking microphotographs of the fungal structures under phase contrast microscopy. Chi-square “goodness of fit” tests were performed in order to compare the effect of different concentrations of sodium bicarbonate on sclerotia formation for each one of the four structure phases. For it was used the VassarStats: Web Site for Statistical Computation (Lowry 2001-2018).

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RESULTS AND DISCUSSION

The presented fungal structures are part of the process of sclerotia formation by S. sclerotiorum, và were microscopically observed before the initiation stage. The sclerotial formation in the control treatment (Figure 1, A-C) began with the proliferation of primary branching of main hyphae, thus, denoting the formation of the sclerotial primordia. As second structure, the hyphal branching became more profuse, and the presence of septa was observed in apical zones (Figure 1, D-F), while the third structure was characterized by presenting small hyphal clusters (Fig. 1, G-I). Finally, in the fourth structure, a massive cluster of hyphae was observed, in which some pigmented cells were visible (Figure 1, J-L). The initiation stage began with the union of several hyphal clusters, và this stage was macroscopically observed when a hyphal conglomerate starts growing on the surface of the culture medium.


Figure 1 Microscopic developmental structures observed during the sclerotial formation of Sclerotinia sclerotiorum after seven days of fungal growth, without KHCO3 (Control). First structure: branching of hyphae, indicated by arrows (A-C); second structure: profuse branching of hyphae (D-F); third structure: clustering of hyphae (G-I); fourth structure: massive sầu clustering of hyphae that build up the sclerotia in the initiation process (J-L). Microphotographs were taken in phase contrast microscopy at 40X magnification. Bars = 10 µm. 


Results showed that the application of KHCO3 had inhibitory effects on the morphology of the sclerotial primordia (Table 1). In fact results from the 50 mM dose were not included on statistical analysis due the complete absence of mycelial growth. Unlikely the treatment without bicarbonate (control) and 2 mM bicarbonate, treatments with all the others concentrations of this chemical compound showed only three developmental structures, in which we noticed that the primordia were irregularly shaped with the formation of loose cells. As the concentration of KHCO3 increased, primordia became smaller & less compact. The increase in the concentration of bicarbonate resulted in growth inhibition of both hyphae and sclerotia (Figure 2 và 3). However, at 2 mM, 4 mM and 6 mM concentrations of KHCO3 only scarce morphological changes were observed.


Table 1 Frequencies of sclerotia on each structure phase. The value on last column indicates the probability of not effects due to sodium bicarbonate exposition for each structure phase (chi square “goodness fit”). 


Tratamientos Concentraciones de bicarbonakhổng lồ de sodio
Fase de la estructura Control 2 mM 4 mM 6 mM 8 mM 10 mM 25 mM Probabilidad
I 6 5 5 6 5 5 7 0.8606
II 5 5 5 5 5 5 0 0.2914
III 4 5 5 6 5 5 0

Figure 2 Microscopic developmental structures observed during the sclerotial formation of Sclerotinia sclerotiorum, after seven days of fungal growth. First (indicated by arrows), second, và third structure of sclerotial development in presence of KHCO3: (A-C) 2 mM, (D-F) 4 mM, và (G-I) 6 mM. Microphotographs were taken in phase contrast microscopy at 40X magnification. Bars = 10 µm. 


Figure 3 Microscopic developmental structures observed during the sclerotial formation of Sclerotinia sclerotiorum, after seven days of fungal growth. First (indicated by arrows), second và third structures of sclerotial development in presence of KHCO3: (A-C) 8 mM, và (D-F) 10 mM. For 25 mM a cellular dischrissiemanby.comanization of the primordium was observed (G-I), in which the second and third structures of sclerotial development were not observed. Microphotographs were taken in phase contrast microscopy at 40X magnification. Bars = 10 μm. 


The most notorious morphological changes were evident after the fungus was exposed khổng lồ concentrations greater than 8 mM of KHCO3. At 10 mM, the presence of primordia was noticed although not very well developed. Despite this, it was possible khổng lồ observe sầu the third structures of development (Figure 3, F), but it was not similar khổng lồ the control by showing irregular formations of the sclerotium in which hyphal clusters were more loose (Figure 1, G-I). At 25 mM concentration, the formation of sclerotia primordia was scarce & dischrissiemanby.comanized (Figure 3, G-I), hence either the second or third structures of the sclerotium initiation could not be completed nor observed (Table 1). Finally, at the 50 mM concentration, no effects were noticed due khổng lồ the absence of fungal growth in this treatment.

In this study, we observed the formation process of sclerotia during their initiation phase in which the four stages of development were identified (Bolton et al., 2006; Saharan & Mehta, 2008). However, the sclerotial formation showed variations depending on the concentration of KHCO3. The inhibitory effect of bicarbonates on the growth of several species of phytopathogenic fungi, especially during postharvest, has been recorded before (Aharoni et al., 1997; Palmer et al., 1997; Bombelli & Wright, 2006; Jabnoun-Khiareddine et al., 2016), & Ordóñez-Valencia et al. (2009) demonstrated that KHCO3 inhibited the growth of S. sclerotiorum in in vitro cultures. The inhibitory effect of bicarbonate salts on fungi was probably due to reduced fungal cell turgor pressure, which resulted in collapse and shrinkage of hyphae (Türkkan et al., 2017).

The formation of sclerotial primordia by S. sclerotiorum was initiated by branching and clustering of hyphae (Figure 1), resulting in a mass of cells that eventually originate mature sclerotia. Similar effects were observed by Smits and Noguera (1988) in the formation of sclerotia of Macrophomimãng cầu phaseolina, which began from hyphal branching và entwinements, besides the increase in kích cỡ of the associate cells & the reduction in kích thước of the sclerotial mass. Townsend và Willets (1954) observed different development patterns (thickening, branching, & septation of main hyphae và their entwinement) in Rhizoctonia solani, Botrytis allii, B. cinerea, và Sclerotium cepivorum.

In the present study, KHCO3 resulted in microscopic morphological changes during early phases of sclerotia development. The increase of bicarbonate concentrations resulted in less profuse và loose hyphal branching, leading khổng lồ the decrement và consequent absence of well-formed sclerotia (Table 1). Igwegbe et al. (1977) reported that the addition of 50 µg mL-1 of 6-metilpurine caused significant reduction in the sclerotia formation by S. rolfsii.

The addition of KHCO3 caused an increase in pH (from 6.5 lớn 8.0) in the culture medium (Ordóñez-Valencia et al., 2009), which resulted in reductions of fungal growth. In this regard, Alexander (1977) mentioned that many fungi grow better under acidic conditions than alkaline, because an acidic environment is not conducive to lớn the existence of either bacteria or actinomycetes, resulting in the monopoly of fungi for utilization of chrissiemanby.comanic substrates (Giri et al., 2005). On the other h&, it has been observed that both growth và development of sclerotia of S. sclerotiorum depkết thúc on the pH và the production of oxalic acid (Rollins và Dickman, 2001; Chen et al., 2004). Neutral or alkaline pH values inhibit the formation of sclerotia, and the production of oxalic acid helps reducing the alkaline pH of the medium, creating more favorable conditions for the development of sclerotia (Rollins and Dickman, 2001).

Although some reports have described the negative effects of bicarbonate on certain plant pathogenic fungi (Bombelli and Wright, 2006; Ordóñez-Valencia et al., 2009), yet the present study is one of the first reports describing inhibitory effects of KHCO3 on S. sclerotiorum during the initial phases of the sclerotia formation as well as on the morphology of sclerotial primordia.

The inhibitory effects of KHCO3 on fungal growth & development may in part be explained by affecting vital biochemical processes such as the biogenesis of either the fungal cell wall and/or the apical wall (Sentandreu et al., 1994; Sideri và Gechrissiemanby.comiou, 2000). Certain antimicrobial compounds cause oxidative sầu bao tay in fungi which may show morphological changes, impaired growth rate, & low content of proteins & ATP (Harel et al., 2005; Marcet-Houben & Gabaldon, 2011). In this regard, the application of KHCO3 may trương mục on the production of reactive sầu oxygen species (ROS) as a response of the bao tay generated by this salt, then, causing alterations on the morphology and development of S. sclerotiorum.

Bicarbonate ions cause alterations in oxidation và nitration reactions in biologicalsystems, regulate pH, và stimulate the production of either reactive nitrogenspecies such as peroxynitrite (ONOO-) or superoxide (O2-)(Knorev et al.,2000; Arai et al.,2005; Lushchak etal., 2009). As a result of oxidative ức chế in combinationwith abiotic factors also have sầu negative effects on the sclerotia formation infilamentous fungi (Gechrissiemanby.comiou etal., 2006). Moreover, Sideri và Gechrissiemanby.comiou (2000) demonstrated that the production ofhydrogene peroxide (H2O2) in S. rolfsii(Typhulaceae) exposed lớn different light and iron conditions; the highestproduction of H2O2 was recorded during early stages offungal growth. However, as sclerotia become mature the hydrogene peroxideproduction decreased. Nevertheless, further research is needed to lớn elucidate theeffects of KHCO3 on either physiological, biochemical or molecularprocesses during fungal morphogenesis.